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human intestinal epithelial c2bbe1 cells  (ATCC)


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    Structured Review

    ATCC human intestinal epithelial c2bbe1 cells
    Human Intestinal Epithelial C2bbe1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+intestinal+epithelial+cells/C2BBe1/pm42320376-78-0-10
    Average 95 stars, based on 448 article reviews
    human intestinal epithelial c2bbe1 cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: 4-Octyl itaconate attenuates radiation-induced intestinal injury associated with ferroptosis inhibition and microbiota rebalance.
    Article Snippet: Radiation-induced intestinal injury (RIII) is a serious and common complication of radiotherapy, and there are currently no effective therapeutic strategies.. This study investigates the protective role of 4-octyl itaconate (4OI), a cell-permeable itaconate derivative, against RIII.. In vitro, 4-OI pretreatment enhanced the viability of irradiated intestinal epithelial cells, reduced reactive oxygen species (ROS) accumulation, and alleviated DNA damage.

    Article Title: Neuroinflammation-Modulating Properties Combining Glutathione, N-Acetylcysteine, and Uridine Monophosphate in a Formulation Supplement: An In Vitro Study.
    Article Snippet: For demyelination induction in 3D EngNT, 200 ng/mL of glial growth factor 2 (GGF, Tebu-Bio, Magenta, Milan, Italy) was added to the same medium [42]. .. Caco-2, human intestinal epithelial cells (ATCC, Manassas, VA, USA) were cultured in Adv DMEM-F12 (GIBCO® ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, 2 mM L-glutamine, and 1% penicillin-streptomycin at 37 ◦C in a 5% CO2 incubator [43]. ..

    Article Title: Neuroinflammation-Modulating Properties Combining Glutathione, N-Acetylcysteine, and Uridine Monophosphate in a Formulation Supplement: An In Vitro Study
    Article Snippet: For demyelination induction in 3D EngNT, 200 ng/mL of glial growth factor 2 (GGF, Tebu-Bio, Magenta, Milan, Italy) was added to the same medium [ ]. .. Caco-2, human intestinal epithelial cells (ATCC, Manassas, VA, USA) were cultured in Adv DMEM-F12 (GIBCO ® ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% FBS, 2 mM L-glutamine, and 1% penicillin-streptomycin at 37 °C in a 5% CO 2 incubator [ ]. ..

    Article Title: Deoxynivalenol-induced apoptosis and IL-1β modulation by fosfomycin in porcine immune and human intestinal epithelial cells.
    Article Snippet: Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium spp., frequently contaminates cereals and animal feed worldwide.. In pigs, one of the most sensitive species, DON primarily exerts immunotoxic effects, including apoptosis of immune cells and dysregulation of cytokine responses, which compromise intestinal integrity and increase susceptibility to infections (Savard et al. 2015).. In humans, DON ingestion is associated with gastrointestinal disturbances, immune modulation, and systemic inflammation (Pinton et al. 2009; Namorado et al. 2024).

    Article Title: Cyanidin and delphinidin inhibit transglutaminase 2 and mitigate inflammatory effects of IFN-γ and TNF-α by molecular interaction.
    Article Snippet: In this assay, TG2-mediated crosslinking of the transglutaminase substrate 5-biotinamidopentylamine (5BP; 500 μmol L−1, 21345, Thermo Fisher Scientific) was detected by streptavidin-HRP as described above. .. Human intestinal epithelial cells (Caco-2; ATCC HTB-37, Manassas, Virginia, USA) were cultured at 37 °C with 5% CO2 and 95% humidity in Dulbeccos Modified Eagle Medium (DMEM; Thermo Fisher Scientific) supplemented with 1% penicillin–streptomycin, 1% non-essential amino acids, 1% sodium pyruvate (all Thermo Fisher Scientific), and 10% heatinactivated fetal bovine serum (FBS; Thermo Fisher Scientific, LOT B2724126RP). .. The culture medium was replaced every two to three days and cells were passaged at 60 to 80% confluence using TrypLE express (Thermo Fisher Scientific).

    Article Title: Divergent roles of Hsp70 chaperones in orthoflavivirus protein secretion and virion formation
    Article Snippet: .. Human embryonic kidney epithelial cells (HEK293T, ATCC CRL-3216), human intestinal epithelial cells (CaCo-2, ATCC HTB-37), human lung epithelial cells (A549, ATCC CCL-185), African green monkey kidney epithelial cells (Vero E6, ATCC CRL-1586) and Syrian golden hamster kidney fibroblasts (BHK-21, ATCC C-13) were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, Taufkirchen, Germany) supplemented with 10% fetal bovine serum (FBS), 1% penicillin (10,000 U/mL)/streptomycin (10 mg/mL) solution and 2 mM L-glutamine solution (Sigma-Aldrich, Taufkirchen, Germany) at 37 °C with 5% CO 2 in a humidified atmosphere. .. Aedes albopictus larvae cells (C6/36, ATCC CRL-1660) were cultivated in Schneiders’ Drosophila Medium (PAN Biotech GmbH, Aidenbach, Germany) supplemented with 10% FBS, 2 mM L-glutamine solution, 1% MEM NEAA (100×) without L-glutamine, 1 mM sodium pyruvate solution and 1% penicillin (10,000 U/mL)/streptomycin (10 mg/mL) (Sigma-Aldrich, Taufkirchen, Germany) at 28 °C.

    Modification:

    Article Title: 4-Octyl itaconate attenuates radiation-induced intestinal injury associated with ferroptosis inhibition and microbiota rebalance.
    Article Snippet: Radiation-induced intestinal injury (RIII) is a serious and common complication of radiotherapy, and there are currently no effective therapeutic strategies.. This study investigates the protective role of 4-octyl itaconate (4OI), a cell-permeable itaconate derivative, against RIII.. In vitro, 4-OI pretreatment enhanced the viability of irradiated intestinal epithelial cells, reduced reactive oxygen species (ROS) accumulation, and alleviated DNA damage.

    Article Title: Cyanidin and delphinidin inhibit transglutaminase 2 and mitigate inflammatory effects of IFN-γ and TNF-α by molecular interaction.
    Article Snippet: In this assay, TG2-mediated crosslinking of the transglutaminase substrate 5-biotinamidopentylamine (5BP; 500 μmol L−1, 21345, Thermo Fisher Scientific) was detected by streptavidin-HRP as described above. .. Human intestinal epithelial cells (Caco-2; ATCC HTB-37, Manassas, Virginia, USA) were cultured at 37 °C with 5% CO2 and 95% humidity in Dulbeccos Modified Eagle Medium (DMEM; Thermo Fisher Scientific) supplemented with 1% penicillin–streptomycin, 1% non-essential amino acids, 1% sodium pyruvate (all Thermo Fisher Scientific), and 10% heatinactivated fetal bovine serum (FBS; Thermo Fisher Scientific, LOT B2724126RP). .. The culture medium was replaced every two to three days and cells were passaged at 60 to 80% confluence using TrypLE express (Thermo Fisher Scientific).

    Article Title: Divergent roles of Hsp70 chaperones in orthoflavivirus protein secretion and virion formation
    Article Snippet: .. Human embryonic kidney epithelial cells (HEK293T, ATCC CRL-3216), human intestinal epithelial cells (CaCo-2, ATCC HTB-37), human lung epithelial cells (A549, ATCC CCL-185), African green monkey kidney epithelial cells (Vero E6, ATCC CRL-1586) and Syrian golden hamster kidney fibroblasts (BHK-21, ATCC C-13) were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, Taufkirchen, Germany) supplemented with 10% fetal bovine serum (FBS), 1% penicillin (10,000 U/mL)/streptomycin (10 mg/mL) solution and 2 mM L-glutamine solution (Sigma-Aldrich, Taufkirchen, Germany) at 37 °C with 5% CO 2 in a humidified atmosphere. .. Aedes albopictus larvae cells (C6/36, ATCC CRL-1660) were cultivated in Schneiders’ Drosophila Medium (PAN Biotech GmbH, Aidenbach, Germany) supplemented with 10% FBS, 2 mM L-glutamine solution, 1% MEM NEAA (100×) without L-glutamine, 1 mM sodium pyruvate solution and 1% penicillin (10,000 U/mL)/streptomycin (10 mg/mL) (Sigma-Aldrich, Taufkirchen, Germany) at 28 °C.

    Expressing:

    Article Title: Deoxynivalenol-induced apoptosis and IL-1β modulation by fosfomycin in porcine immune and human intestinal epithelial cells.
    Article Snippet: Deoxynivalenol (DON), a trichothecene mycotoxin produced by Fusarium spp., frequently contaminates cereals and animal feed worldwide.. In pigs, one of the most sensitive species, DON primarily exerts immunotoxic effects, including apoptosis of immune cells and dysregulation of cytokine responses, which compromise intestinal integrity and increase susceptibility to infections (Savard et al. 2015).. In humans, DON ingestion is associated with gastrointestinal disturbances, immune modulation, and systemic inflammation (Pinton et al. 2009; Namorado et al. 2024).



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    Image Search Results


    Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

    Journal: Nutrients

    Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

    doi: 10.3390/nu18091393

    Figure Lengend Snippet: Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

    Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

    Techniques: Viability Assay, Control

    Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

    Journal: Nutrients

    Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

    doi: 10.3390/nu18091393

    Figure Lengend Snippet: Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

    Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

    Techniques: Concentration Assay, Control

    Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

    Journal: Nutrients

    Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

    doi: 10.3390/nu18091393

    Figure Lengend Snippet: Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

    Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

    Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Derivative Assay, Control